|
KMID : 0371620020170020133
|
|
Journal of Wonkwang Medical Science
2002 Volume.17 No. 2 p.133 ~ p.147
|
|
|
The Protective Effects of Morphine on Glutamate-induced Death in C6 glial Cells
|
|
Choi Deok-Hwa
Lee Cheol
Ann Jin-Young
|
|
|
Abstract
|
|
|
|
This study was designed to investigate the effect of morphine on the glutamate-induced toxicity of rat C6 glial cells. Glutamate increased the death of C6 glial cells in a time and dose-dependent manner, which was revealed as apoptosis characterized by genomic DNA fragmentation. Caspase family cysteine proteases including caspase-3, -8 and -9 proteases were not activated in C6 glial cells by glutamate. However, cleavage of PARP was occurred at 12 hr after treatment of glutamate. Glutamate also activated the transcriptional activators including NF-_KB and AP-1 in C6 glial cells. Glutamate-induced cytotoxicity was prevented by morphine and antioxidants such as NAC and GSH, whereas it was not inhibited by MK-801, an antagonist of glutamate receptor NMDA and opioid agonists including DAMGO, DPDPE, and U69593. A morphine antagonist naloxone did not inhibit the protective effect of morphine on cytotoxicity by glutamate but it prevent the death of C6 glial cells. Glutamate significantly decreased the intracellular contents of GSH and increased the generation of H_2O_2 in C6 glial cells. On the contrary, morphine significantly inhibited the depletion of GSH as well as generation of H_2O_2 in C6 glial cells by glutamate.
|
|
|
KEYWORD
|
|
|
Morphine, glutamate, C6 glial cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|